Protein Identification and Quantitation

Protein identification

• Protein id's from samples such as gel bands, IP pulldowns, or cell lysates

• Performed by LC-MS/MS using Thermo Orbitrap mass spectrometers

 

Protein Quantitation - Untargeted Relative Quantitation

• ITRAQ/TMT labelling - for improved accuracy

• Label Free - for low cost analysis of many samples

• SILAC - metabolic incorporation of label 

 

Protein Quantitation - Targeted

• Targeted protein quantitation using table isotope labeled internal standards and LC-MRM mass spectrometry