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Protein Identification and Quantitation

Protein identification

  • Protein id's from samples such as gel bands, IP pulldowns, or cell lysates

  • Performed by LC-MS/MS using Thermo Orbitrap mass spectrometers

 

Protein Quantitation - Untargeted Relative Quantitation

  • ITRAQ/TMT labelling - for improved accuracy

  • Label Free - for low cost analysis of many samples

  • SILAC - metabolic incorporation of label 

 

Protein Quantitation - Targeted

  • Targeted protein quantitation using table isotope labeled internal standards and LC-MRM mass spectrometry