Purine/pyrimidine Synthesis Pathway

Assay Overview

Measurement of metabolic precursors and intermediates of IMP and UMP synthesis using LC-MRM/MS, complementary with high-resolution LC-MS, for absolute quantitation (with standards) and relative quantitation for the metabolites without standards commercially available.

Pathways:

Nucleotide synthesis, IMP synthesis, UMP synthesis

Minimum Amount Required

Tissue 20 mg
Cell 5x10^6
Blood/plasma/serum TBD
Urine N/A
Plant TBD

Compound Classes

Academic Pricing

$400 1st sample; $120 each additional sample

Industry Pricing

$800 1st sample; $240 each additional sample

Nucleotide synthesis precursors, intermediates and end products

Compounds

Ribose-5 phosphate
Phosphoribosyl pyrophosphate (PRPP)
Phosphoribosylamine
Glycinamide ribonucleotide (GAR)
Formylglycinamide Ribotide (FGAR)
Formylglycinamidine Ribotide (FGAM)
5-aminoimidazole ribonucleotide (ribotide) (AIR)
Carboxyaminoimidazole ribonucleotide (CAIR)
N-Succinyl-5-aminoimidazole-4-carboxamide (SAICA)
5-aminoimidazole-4-Carboxamide Riboside (AICAR)
N-Succinyl-5-aminoimidazole-4-carboxamide Riboside (SAICAR)
5-Formylamino-4-imidazolecarboxamide Ribonucleotide (FAICAR)
IMP
Carbamoyl phosphate
Carbamoyl aspartate
Dihydrorotate
Orotate
Orotidine-5 phosphate
UMP