Mevalonate Pathway and Isoprenoids/Cholesterol Synthesis

Detection and quantitation of isoprenyl phosphate intermediates, isoprenoids (ubiquinones, dolichols, vitamin K2 and squalene, etc.), cholesterol and other sterols by UPLC-MRM/MS and UPLC-FTMS

Minimum Amount Required

Tissue 50 mg
Cell 5x10^6
Blood/plasma/serum 100 µL
Urine N/A
Plant N/A
Feces 50 mg
DBS 30 µL

Academic Price

$400 1st sample; $180 each additional sample

Industry Price

$800 1st sample; $360 each additional samples

Pathways

Mevalonate pathway, isoprenoid synthesis cholesterol synthesis

Compound Classes

Isoprenyl phosphates and pyrophosphates, HMG-CoA, isoprenoids, ubiquinones, dolichols, squalene, oxidosqualene, menaquinones (vitamin Ks), sterols and cholesterol

Compounds

Acetyl-CoA
Acetoacetyl-CoA
3-Hydroxy-3-methylglutaryl CoA (HMG-CoA)
Mevalonic acid
Mevalonic acid 5-phosphate
Mevalonic acid 5-pyrophosphate
Isopentenyl pyrophosphate
Dimethylallyl pyrophosphate
Geranyl pyrophosphate
Farnesyl pyrophosphate
Geranylgeranyl pyrophosphate
Ubiquinones ---CoQ6, CoQ8, CoQ9, CoQ10, CoQ11, CoQ12
Dolichols ( 13 to 21 isoprene units)
Polyprenols (13 to21 isoprene units)
Squalene
2,3-oxidosqualene
Menoquinones (vitamin Ks, e.g., MK-4)
Lanosterol
Zymosterol
7-Dehydrodesmosterol
Desmosterol
Dihydrolanosterol
Zymostenol
Lathostenol
7-Dehydrocholesterol (provitamin D3)
Cholesterol