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Central Carbon Metabolism

Assay Overview

Quantification of glucose, sugar phosphates, CoAs, enzyme co-factors, TCA cycle carboxylic acids, phosphocarboxylic acids and major nucleotides involved in Central Carbon Metabolism (CCM) and glucosamine metabolism by UPLC-MRM/MS

Pathways:

Central carbon metabolism, glucose metabolism, hexose metabolism, glucosamine metabolism, TCA cycle, glycolysis, pentose phosphate pathway, gluconeogenesis, glyoxylate shunt, GABA shunt, shikimate pathway

Minimum Amount Required

Tissue 10 mg
Cell 2x10^6
Blood/plasma/serum 100 µL
Urine N/A
Plant 50 mg
Feces N/A
DBS 50 µL
Cell medium 100 µL

Compound Classes

Academic Pricing

$400 1st sample; $180 each additional sample

Industry Pricing

$800 1st sample; $360 each additional sample

Sugars, sugar phosphates, nucleotides, acetyl CoAs, enzyme cofactors, NAD(P)/NAD(P)H, carboxylic acids, phosphocarboxylic acids, nucleotide sugars, glucosamine phosphates, galactosamine phosphates

Compounds

Glucose
Ribose
Dihydroxyacetone-P (DHAP)
Phosphoenolpyruvate (PEP)
Fructose-6-P
Fructose-1-P
Glucose-1-P
Glucose-6-P
Mannose-6-P
Glucose-1,6-bisP
Fructose-1,6-bisP
Ribose-5-P
Ribose-1-P
Ribulose-5-P
Xybulose-5P
Ribulose-1,5-bisP
Ribose-1,5-bisP
Glycerate-1,3-bisP
Glycerate-2,3-bisP
Glycerate-3P
Glycerate-2P
Shikimate-3P
Glycerol-3P
6-Phosphogluconate (6PG)
6-Phosphogluconone
Sedoheptulose-7P
Erythrose-4-P
Glyceraldehyde-3-P
Acetyl-CoA
HS-CoA
Succinyl-CoA
Malonyl-CoA
Acetoacetyl-CoA
NAD+
NADH
NADP+
NADPH
FAD
FMN
ATP
ADP
AMP
GTP
GMP
GDP
UMP
UDP
UTP
cAMP
cGMP
ADP-glucose
GDP-glucose
GDP-fucose
Glucosamine 6-P
N-Acetyl-glucosamine 1-P
N-Acetyl-glucosamine
UDP-glucose
UDP-N-acetylglucosamine (UDP-GlcNAc)
Phosphocreatine
Deoxyribose 1-phosphate
Acetoacetic acid
Methylcitric acid
Glycolic acid
Lactic acid
Pyruvic acid
Oxaloacetic acid
Malic acid
Succinic acid
Fumaric acid
Citric acid
α-Ketogluteric acid
α-Hydroxyglutaric acid
Aconitic acid
Isocitric acid
Methylcitric acid
Citramalic acid
Acetoacetic acid
Citraconic acid
β-Hydroxybutyric acid
Glucaric acid
Gluconic acid
Glyceric acid

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