Sample Conditions -
Ideal and Absolute Limitations
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+ ABC is ammonium bicarbonate NH4HCO3
*always include sample documentation in the package containing
the sample
Please contact the Proteomics Centre for suggestions if your
sample is looking less than ideal – these are only meant
to be guidelines to help us get the best possible results.
Please feel free to contact us by phone or by
email if you have any questions about sample conditions. Our
sample preparation technician Monica Elliot would be delighted
to hear from you and help you avoid common sample pitfalls
that are easily fixed.
The values in this chart are based on combined sources including:
Applied Biosystems and Amersham protocols, Smolka et al.(2001)
Optimization of the Isotope-Coded Affinity Tag-Labeling Procedure
for Quantitative Proteome Analysis. Anal.Biochem. 297, 25-31.,
and empirical determinations within our facility.
IMPORTANT NOTE:
Please remember to declare absolutely everything in your sample
on the sample submission form including solvents, salts, detergents,
denaturing agents, chaotropes, and any other substances. We
will not be responsible for services that did not work because
of interfering components of the sample of which we were not
made aware, nor will we begin work on a sample that is received
without documentation. It is also beneficial to us if you
could describe the state of the sample before it left your
lab ie: soluble in solution, cell pellet, lyophilized, solvent
precipitated etc as well as protein concentration or number
of cells. Often sample that was soluble when it left your
lab will arrive with precipitate at our facility and it is
important for us to know how much room we have before detergent
and other limitations are reached. It is often advantageous
to send an insoluble sample with no detergents or reducing
agents added rather than a sample that has already been saturated
with urea and loaded with detergent to make it soluble.
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