Accurate quantitation of standard peptides used for quantitative proteomics.
Proteomics (2009 Aug;9(15):3939-44.)
Bordeerat, NK, Georgieva, NI, Klapper, DG, Collins, LB, Cross, TJ, Borchers, CH, Swenberg, JA and Boysen, G.
Bordeerat, NK, Georgieva, NI, Klapper, DG, Collins, LB, Cross, TJ, Borchers, CH, Swenberg, JA and Boysen, G.
MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 muM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
