MALDI Imaging Mass Spectrometry Workshop, Victoria BC

brain stripe

March 1st-2nd, 2010
A two-day workshop hosted by the University of Victoria Genome BC Proteomics Centre and instructed by Pierre Chaurand, Ph.D. (University of Montreal)

maldi workshop

Eager students watch as Dr. Pierre Chaurand demonstrates H&E histological staining on rat spinal cord tissue.

Imaging MS is a MALDI-based mass spectrometry technology that allows determination of the spatial distribution of biomolecules such as lipids, peptides and proteins, and xenobiotics such as administered pharmaceuticals present in tissue sections. The purpose of this two-day workshop is to familiarize with the principles and methodologies involved in MALDI imaging MS through a series of lectures and hands-on demonstrations.

Day 1: Monday March 1st To understand the principle of MALDI and imaging MS. This will be achieved via two lectures:
a) Morning (9:30-11:30): Introduction to MALDI MS. Principle, instrumentation (TOF, Q-TOF, FT/ICR, ion trap, triple quadrupole). The strengths and limitations of MALDI MS will also be addressed.
b) Afternoon (13:00-15:00): Introduction to MALDI imaging MS. Principle, tissue handling and sectioning, tissue section rinsing and staining, analytical strategies, matrix deposition approaches, in-situ chemistries, data acquisition, image visualization and analysis, statistical analyses.

Day 2: Tuesday March 2nd Demonstration and hands-on learning of imaging MS. In this full day course, most of the aspects seen during Day 1 will be demonstrated. (9:00-16:00)
• tissue handling and sectioning (cryostat),
• section rinsing and staining,
• matrix deposition (ImagePrep),
• image acquisition by MALDI-TOF MS (UltraFlex),
• image reconstruction, visualization and analysis (FlexImaging, ClinProTools).

Location: Vancouver Island Technology Park (VITP) , Victoria BC link to map
Click here for more information about transportation and accommodation in Victoria

Contact: leanne@proteincentre.com for more information and registration

Participants can also sign up for remote access to lectures on Day 1 via WebEx online session.
Registration for Day 1 - FREE
Registration for Day 2 - registration closed, workshop full
DEADLINE for registration is February 22, 2010

Sponsors:

bruker BCPN